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Plant Focus

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A guest post by Matt Candeias, host of the In Defense of Plants podcast and blog

Micropropagation of Quercus arbutifolia

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Qiansheng Li, Min Deng and Mengmeng Gu

Published May 2019 in International Oaks No. 30: 217–224

Abstract

Quercus arbutifolia, a native and vulnerable species of section Cyclobalanopsis with only five known populations in China, is suffering severe decline in population size. To increase the number of individuals for further conservation, a micropropagation protocol is investigated. Nodal segments were cut from 2-year-old-seedling shoots as explants for aseptic initiation culture. Woody plant medium (WPM) with 0.5 mg/L BA was optimal for budding initiation to produce initial shoots for further subculture of proliferation. WPM with 0.5 mg/L BA and 0.02 mg/L IBA is suitable for subculture that can vigorously induce shoot regeneration. For rooting in vitro, WPM with 0.1 mg/L IBA and activated charcoal was suitable. Potting mix with 50% peat and 50% coarse perlite was recommended for greenhouse acclimatization.

Keywords

Quercus arbutifolia, tissue culture, conservation, propagation

References

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